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Image Search Results
Journal: Nanomedicine : nanotechnology, biology, and medicine
Article Title: Nanoparticles functionalised with an anti-platelet human antibody for in vivo detection of atherosclerotic plaque by magnetic resonance imaging.
doi: 10.1016/j.nano.2014.12.006
Figure Lengend Snippet: Figure 1. Flow cytometry and optical fluorescence microscopy assays performed on PFA-activated platelets. Incubations with AP-2 murine monoclonal antibody or rIgG4 TEG4 human monoclonal antibody (A), and TEG4-VUSPIO contrast agent or IgG cont-VUSPIO (B) were followed by a second incubation with species-specific secondary antibodies labelled with Alexa Fluor 488 or anti-dextran antibodies labelled with FITC.The labelling of platelets in flow cytometry (B, i, m) and optical microscopy (B, j) with the TEG4-VUSPIO conjugate is similar to the labelling with rIgG4 TEG4 human antibody (A, e, f). Bar: 5 μm.
Article Snippet: In flow cytometry experiments, aliquots of the cell samples were further incubated 30 minutes with secondary Alexa Fluor 488 goat anti-human H + L (
Techniques: Flow Cytometry, Fluorescence, Microscopy, Incubation