primary murine antidextran antibody no. 14533 Search Results


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STEMCELL Technologies Inc primary murine antidextran antibody no. 14533
Primary Murine Antidextran Antibody No. 14533, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc mouse anti-dextran-fitc
Mouse Anti Dextran Fitc, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno fluorescein isothiocyanate fitc mouse anti dextran antibodies
Figure 1. Flow cytometry and optical fluorescence microscopy assays performed on PFA-activated platelets. Incubations with AP-2 murine monoclonal antibody or rIgG4 TEG4 human monoclonal antibody (A), and TEG4-VUSPIO contrast agent or IgG cont-VUSPIO (B) were followed by a second incubation with species-specific secondary antibodies labelled with Alexa Fluor 488 or anti-dextran antibodies labelled with <t>FITC.The</t> labelling of platelets in flow cytometry (B, i, m) and optical microscopy (B, j) with the TEG4-VUSPIO conjugate is similar to the labelling with rIgG4 TEG4 human antibody (A, e, f). Bar: 5 μm.
Fluorescein Isothiocyanate Fitc Mouse Anti Dextran Antibodies, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno alexa fluor 488 goat anti human h l
Figure 1. Flow cytometry and optical fluorescence microscopy assays performed on PFA-activated platelets. Incubations with AP-2 murine monoclonal antibody or rIgG4 TEG4 human monoclonal antibody (A), and TEG4-VUSPIO contrast agent or IgG cont-VUSPIO (B) were followed by a second incubation with species-specific secondary antibodies labelled with Alexa Fluor 488 or anti-dextran antibodies labelled with <t>FITC.The</t> labelling of platelets in flow cytometry (B, i, m) and optical microscopy (B, j) with the TEG4-VUSPIO conjugate is similar to the labelling with rIgG4 TEG4 human antibody (A, e, f). Bar: 5 μm.
Alexa Fluor 488 Goat Anti Human H L, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. Flow cytometry and optical fluorescence microscopy assays performed on PFA-activated platelets. Incubations with AP-2 murine monoclonal antibody or rIgG4 TEG4 human monoclonal antibody (A), and TEG4-VUSPIO contrast agent or IgG cont-VUSPIO (B) were followed by a second incubation with species-specific secondary antibodies labelled with Alexa Fluor 488 or anti-dextran antibodies labelled with FITC.The labelling of platelets in flow cytometry (B, i, m) and optical microscopy (B, j) with the TEG4-VUSPIO conjugate is similar to the labelling with rIgG4 TEG4 human antibody (A, e, f). Bar: 5 μm.

Journal: Nanomedicine : nanotechnology, biology, and medicine

Article Title: Nanoparticles functionalised with an anti-platelet human antibody for in vivo detection of atherosclerotic plaque by magnetic resonance imaging.

doi: 10.1016/j.nano.2014.12.006

Figure Lengend Snippet: Figure 1. Flow cytometry and optical fluorescence microscopy assays performed on PFA-activated platelets. Incubations with AP-2 murine monoclonal antibody or rIgG4 TEG4 human monoclonal antibody (A), and TEG4-VUSPIO contrast agent or IgG cont-VUSPIO (B) were followed by a second incubation with species-specific secondary antibodies labelled with Alexa Fluor 488 or anti-dextran antibodies labelled with FITC.The labelling of platelets in flow cytometry (B, i, m) and optical microscopy (B, j) with the TEG4-VUSPIO conjugate is similar to the labelling with rIgG4 TEG4 human antibody (A, e, f). Bar: 5 μm.

Article Snippet: In flow cytometry experiments, aliquots of the cell samples were further incubated 30 minutes with secondary Alexa Fluor 488 goat anti-human H + L (Jackson Immuno Research Laboratories) (1/20), goat anti-mouse H + L (Life Technologies, Saint-Aubin, France) (1/20) and fluorescein isothiocyanate (FITC) mouse anti-dextran antibodies (Stem-Cell Technologies, Grenoble, France) (1/5) for detection of respectively, the functionalising primary antibody (rIgG4 TEG4), murine AP-2 antibody and the dextran coating of antibody-conjugated VUSPIO nanoparticles.

Techniques: Flow Cytometry, Fluorescence, Microscopy, Incubation